DNA Macroarray for Identification and Typing of Staphylococcus aureus Isolates
نویسندگان
چکیده
منابع مشابه
Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates.
A total of 258 bovine-associated Staphylococcus aureus isolates from the United States, Chile, and the United Kingdom, plus the reference isolate S. aureus Newbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST). A collection of previously characterized United Kingdom isolates were also included in the analysis. The results demonstrated that MLST is suitable for the diff...
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A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventiona...
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BACKGROUND We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S. aureus. METHODOLOGY/PRINCIPAL FINDINGS A novel approach of IS-typing was performed in which bina...
متن کاملIdentification of Virulence Genes in Staphylococcus aureus Isolates Segregated from Children’s Wounds
Background: Staphylococcus aureus is an opportunistic pathogenic bacterium that can provide invasive conditions by secreting various toxins. It is involved in the development of skin infections, such as swollen and ulcers. Today, one of the main problems is the treatment of Staphylococcus aureus resistant strains. The aim of the current study was to detect virulence factors and antibiotic re...
متن کاملComparison of traditional and molecular methods of typing isolates of Staphylococcus aureus.
Fifty-nine Staphylococcus aureus isolates and 1 isolate of Staphylococcus intermedius were typed by investigators at eight institutions by using either antibiograms, bacteriophage typing, biotyping, immunoblotting, insertion sequence typing with IS257/431, multilocus enzyme electrophoresis, restriction analysis of plasmid DNA, pulsed-field or field inversion gel electrophoresis, restriction ana...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2004
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.42.5.2054-2064.2004